High-performance liquid chromatographic determination of cardenolides in Digitalis leaves after solid-phase

¹Institut für Pharmazeutische Biologie, Philipps-Universität, Marburg/Lahn, Germany.

|February 5, 1993

Abstract:

For HPLC analysis of cardenolide glycosides of Digitalis lanata the separation of the main compounds from other constituents is useful. An improved method for doing this, based on solid-phase extraction on a C18 modified poly(styrene-divinylbenzene) polymer, is described. The presented assay permits quantitative estimation of more than 50 cardenolides in about 2 mg of dried leaf powder of Digitalis lanata with high speed and accuracy.

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Abstract:

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High-performance liquid chromatographic determination of cardenolides in Digitalis leaves after solid-phase