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Inhibition of interphotoreceptor retinoid-binding protein-induced uveitis by monoclonal antibodies
L Atalla1, M Linker-Israeli, G Pararajasegaram
1Doheny Eye Institute, Los Angeles, CA 90033.
Immunization of Lewis rats with interphotoreceptor retinoid-binding protein (IRBP) resulted in development of uveoretinitis in 100% (12/12) of the animals so injected. In the present study, we attempted immunotherapy of this intraocular inflammation using the monoclonal antibodies anti-I-A and anti-I-E. The antibodies were injected 1 day before and on days 1 and 2 after IRBP sensitization, and the animals were killed 16 days later. Anti-I-A treatment inhibited the disease in 83% (10/12) of the animals, while anti-I-E antibodies resulted in inhibition of the disease in 67% (8/12) of the animals. All rats injected with preimmune IgG (control animals) developed uveoretinitis. The anti-I-A and anti-I-E antibodies exerted a specific suppressor effect on the immune response of these animals. Thus, anti-I-A and anti-I-E antibodies administered at the time of antigen priming abolished the immune function and inhibited the development of autoimmune uveitis.
Immunization of Lewis rats with interphotoreceptor retinoid-binding protein (IRBP) resulted in development of uveoretinitis in 100% (12/12) of the animals so injected. In the present study, we attempted immunotherapy of this intraocular inflammation using the monoclonal antibodies anti-I-A and anti-I-E. The antibodies were injected 1 day before and on days 1 and 2 after IRBP sensitization, and the animals were killed 16 days later. Anti-I-A treatment inhibited the disease in 83% (10/12) of the animals, while anti-I-E antibodies resulted in inhibition of the disease in 67% (8/12) of the animals. All rats injected with preimmune IgG (control animals) developed uveoretinitis. The anti-I-A and anti-I-E antibodies exerted a specific suppressor effect on the immune response of these animals. Thus, anti-I-A and anti-I-E antibodies administered at the time of antigen priming abolished the immune function and inhibited the development of autoimmune uveitis.