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Sustained decrease in brain regional blood flow after microsphere embolism in rats

K Miyake1, S Takeo, H Kaijihara

  • 1Department of Pharmacology, Tokyo College of Pharmacy, Japan.

Stroke
|March 1, 1993
PubMed
Summary
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This study demonstrates that microsphere embolism in rats causes sustained reductions in cerebral blood flow and leads to widespread brain infarction. These findings are crucial for understanding and potentially preventing ischemic brain disease.

Area of Science:

  • Neuroscience
  • Cerebrovascular Research
  • Pathophysiology

Background:

  • Developing models for sustained cerebral ischemia is vital for understanding ischemic brain disease.
  • Microsphere embolism offers a method to study prolonged reductions in cerebral blood flow.

Purpose of the Study:

  • To investigate the pathophysiological effects of sustained cerebral ischemia and infarction induced by microsphere embolism in rats.
  • To analyze changes in regional cerebral blood flow and infarct development over 28 days.

Main Methods:

  • Rats underwent internal carotid artery injection of microspheres to induce embolism.
  • Cerebral blood flow was measured using the hydrogen clearance method over 28 days.
  • Infarct areas were quantified using triphenyltetrazolium chloride and hematoxylin and eosin staining.

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Main Results:

  • Microsphere embolism caused a sustained decrease in cortical and striatal blood flow in the embolized hemisphere.
  • Hippocampal blood flow was initially reduced but tended to recover.
  • Widespread necrosis was observed in multiple brain regions of the embolized hemisphere by day 3, persisting throughout the study.

Conclusions:

  • Microsphere embolism effectively induces sustained regional cerebral hypoperfusion and cerebral infarction in rats.
  • This model is suitable for studying the long-term consequences of cerebral ischemia and potential therapeutic interventions.