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Improved colorimetric method for cholinesterase activity

B V Venkataraman1, M A Rani, C Andrade

  • 1Department of Pharmacology, St. John's Medical College, Bangalore.

Indian Journal of Physiology and Pharmacology
|January 1, 1993
PubMed
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This study introduces a refined colorimetric method for measuring cholinesterase activity. Using specific substrates, butyrylthiocholine iodide and acetylthiocholine iodide, improves the accuracy of pseudocholinesterase and total cholinesterase estimations.

Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Cholinesterase activity is crucial for neurotransmission and is often measured clinically.
  • Existing colorimetric methods may lack specificity in distinguishing between enzyme types.

Purpose of the Study:

  • To develop a modified colorimetric assay for accurate estimation of total and pseudocholinesterase activity.
  • To enhance the specificity of cholinesterase assays by employing distinct substrates for different enzyme types.

Main Methods:

  • A modified colorimetric method was developed using acetylthiocholine iodide for total cholinesterase and butyrylthiocholine iodide for pseudocholinesterase.
  • Assays were performed on whole blood and serum samples to compare substrate efficacy.

Main Results:

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  • Pseudocholinesterase activity measured with acetylthiocholine iodide was significantly higher than with butyrylthiocholine iodide (P < 0.0001).
  • This suggests acetylthiocholine iodide may interact with both acetylcholinesterase and pseudocholinesterase, while butyrylthiocholine iodide is more specific for pseudocholinesterase.

Conclusions:

  • Butyrylthiocholine iodide and acetylthiocholine iodide are recommended as specific substrates for pseudocholinesterase and total cholinesterase determination, respectively.
  • The modified dual-substrate method offers increased validity, simplicity, and speed for cholinesterase activity measurement.