Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CBEIA: programs for simulation of ELISA experiments and affinity determination

R W Glaser1

  • 1Department of Medical Immunology, Humboldt University Berlin, Medical School (Charité), Germany.

Journal of Immunological Methods
|March 15, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The intracellular bacteria of the cockroach in relation to symbiosis.

The Journal of parasitology·2010
Same author

EXPERIMENTS ON THE VISIBILITY OF THE POLYHEDRAL VIRUSES.

The Journal of experimental medicine·2009
Same author

ON THE ISOLATION, CULTIVATION AND CLASSIFICATION OF THE SO-CALLED INTRACELLULAR "SYMBIONT" OR "RICKETTSIA" OF PERIPLANETA AMERICANA.

The Journal of experimental medicine·2009
Same author

METHODS FOR THE PURE CULTURE OF CERTAIN PROTOZOA.

The Journal of experimental medicine·2009
Same author

CULTIVATION AND CLASSIFICATION OF "BACTEROIDES," "SYMBIONTS," OR "RICKETTSIAE" OF BLATTELLA GERMANICA.

The Journal of experimental medicine·2009
Same author

BIOCHEMICAL STUDIES ON THE VIRUS AND THE INCLUSION BODIES OF SILKWORM JAUNDICE.

The Journal of experimental medicine·2009
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Two new computer programs, CBEIA-C and CBEIA-S, enhance enzyme immunoassay analysis. They offer a more reliable method for determining antibody-antigen affinity compared to traditional linearized plots.

Area of Science:

  • Biochemistry
  • Computational Biology
  • Immunology

Background:

  • Enzyme immunoassays (EIAs) are widely used for detecting and quantifying antigens and antibodies.
  • Accurate determination of binding affinities is crucial for understanding molecular interactions in EIAs.
  • Traditional methods for affinity determination using linearized plots can be unreliable.

Purpose of the Study:

  • To present two novel computer programs, CBEIA-C and CBEIA-S, for analyzing EIA results.
  • To provide a more robust method for determining antibody-antigen affinity.
  • To demonstrate the utility of these programs in EIA data analysis.

Main Methods:

  • CBEIA-C simulates competitive EIAs involving immobilized and soluble antibodies or antigens.
  • CBEIA-S simulates ternary complex formation in EIAs.

Related Experiment Videos

  • Both programs utilize simulation-based approaches for data analysis.

    • Main Results:

    • The programs offer a more reliable basis for affinity determination than conventional linearized plots.
    • Simulation results provide a deeper understanding of EIA reaction kinetics.
    • Application of the programs leads to more accurate affinity constants.

    Conclusions:

    • CBEIA-C and CBEIA-S represent significant advancements in EIA data analysis.
    • These programs facilitate more precise affinity measurements in immunological studies.
    • The simulation approach overcomes limitations of traditional linearization methods.