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Intermolecular mRNA-rRNA hybridization and the distribution of potential interaction regions in murine 18S rRNA

O V Matveeva1, S A Shabalina

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

Nucleic Acids Research
|February 25, 1993
PubMed

Insights

Murine ribosomal RNA (rRNA) can hybridize with messenger RNA (mRNA) from oncogenes. Specific 18S rRNA regions, termed clinger-fragments, show universal binding potential with mRNA, suggesting a role in translation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ribosomal RNA (rRNA) plays a crucial role in protein synthesis.
  • Messenger RNA (mRNA) carries genetic information from DNA to ribosomes.
  • Oncogenes like p53, c-myc, and c-mos are critical in cell cycle regulation.

Purpose of the Study:

  • To investigate the intermolecular hybridization capabilities of murine 18S rRNA and 28S rRNA with specific oncogene mRNAs.
  • To identify potential regions on rRNA that interact with mRNA.
  • To explore the functional significance of these interactions in biological processes.

Main Methods:

  • Intermolecular hybridization experiments were conducted using murine 18S rRNA, 28S rRNA, and mRNAs from p53, c-myc, and c-mos genes.
  • Computer analysis was employed to identify complementary sequences and potential hybridization sites (clinger-fragments) within rRNA and mRNA.
  • Sequence analysis compared oligonucleotide frequencies in mRNA and intron sequences.

Main Results:

  • Stable hybrid structures were formed between murine 18S rRNA/28S rRNA and oncogene mRNAs (p53, c-myc, c-mos).
  • Both 5'-untranslated and coding regions of p53 mRNA showed interaction fragments with rRNA.
  • 18S rRNA contains 'clinger-fragments' complementary to common mRNA oligonucleotides, with universal distribution across 100+ murine mRNA sequences.
  • Oligonucleotides complementary to clinger-fragments were significantly more frequent in mRNA than in introns or random sequences.

Conclusions:

  • Murine rRNA, particularly 18S rRNA, possesses specific regions ('clinger-fragments') capable of universal binding with mRNA.
  • These findings suggest a potential role for clinger-fragments in facilitating mRNA binding during translation.
  • The interaction highlights a conserved mechanism potentially involved in gene regulation and protein synthesis.

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