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Human T cell lines and clones respond to IL-9
F A Houssiau1, J C Renauld, M Stevens
1Experimental Medicine Unit, University of Louvain, Belgium.
Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1993
Summary
Interleukin-9 (IL-9) stimulates proliferation in activated human T cells, including CD4+, CD8+, and tumor-specific cytotoxic T lymphocytes (CTLs). Freshly isolated T cells require prior activation to respond to IL-9.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Interleukin-9 (IL-9) is a cytokine involved in immune responses.
- Previous studies, primarily in murine models, suggested limited effects of IL-9 on T cell proliferation.
Purpose of the Study:
- To investigate the activity of IL-9 on human T cells.
- To determine the conditions under which human T cells respond to IL-9.
Main Methods:
- Human T cell lines and clones were generated from peripheral blood mononuclear cells (PBMC).
- Cells were stimulated with PHA, IL-2, and irradiated allogeneic PBMC.
- IL-9 receptor (IL-9R) expression was assessed at the RNA level.
- T cell proliferation was measured in response to IL-9, with and without prior activation.
Main Results:
- Human T cell lines and clones expressed IL-9R mRNA.
- Both CD4+ and CD8+ T cells, as well as tumor-specific cytotoxic T lymphocytes (CTLs), proliferated in response to IL-9.
- Freshly isolated T cells did not proliferate with IL-9, but showed a significant response after 10 days of activation.
- IL-9's proliferative effect on human T cells was broader than observed in murine models.
Conclusions:
- Human T cells possess a functional IL-9 receptor, enabling IL-9 to induce proliferation.
- Proliferative responses to IL-9 in human T cells are dependent on prior T cell activation.
- IL-9 exhibits a wider range of activity on human T cells than previously understood from murine studies.