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Survey on spontaneous systemic amyloidosis in aging mice
1Huntingdon Research Centre Ltd., England.
Arzneimittel-Forschung
|February 1, 1993
Summary
Systemic amyloidosis is common and age-related in CD-1 mice, affecting multiple organs and contributing to mortality. This spontaneous condition was more prevalent in CD-1 mice compared to other strains studied.
Area of Science:
- Veterinary Pathology
- Toxicology
- Aging Research
Background:
- Systemic amyloidosis is a condition characterized by the extracellular deposition of amyloid proteins.
- Understanding the incidence and characteristics of spontaneous amyloidosis in research models is crucial for interpreting study outcomes.
- CD-1 mice are a widely used strain in preclinical research, making their disease incidence data valuable.
Purpose of the Study:
- To report the incidence of spontaneous systemic amyloidosis in CD-1 mice over a 15-year period.
- To characterize the affected organs and contributing factors to mortality in aging CD-1 mice.
- To compare the incidence of amyloidosis in CD-1 mice with other common laboratory strains.
Main Methods:
- A retrospective survey of visceral organs, central and peripheral nervous tissues, bone, and bone marrow from CD-1 mice.
- Histopathological examination of tissues for amyloid deposition.
- Staining techniques including Congo Red, Oil Red O, and Alcian blue to confirm amyloid characteristics.
- Comparison of incidence rates with other mouse strains (B6C3F, CFLP, MAGF: TIF).
Main Results:
- Amyloidosis was observed in various organs including the stomach, heart, intestines, kidney, liver, spleen, thyroid, parathyroid, adrenals, salivary glands, and ovaries.
- No significant difference in incidence was noted between male and female CD-1 mice.
- Amyloid deposition was extracellular, age-related, and spontaneous, staining positively with Congo Red, Oil Red O, and Alcian blue.
- Amyloidosis incidence in CD-1 mice was higher compared to B6C3F, CFLP, and MAGF: TIF strains.
- Spontaneous systemic amyloidosis was a major contributor to death in aging CD-1 mice.
Conclusions:
- Spontaneous systemic amyloidosis is a significant, age-related finding in CD-1 mice.
- The condition affects a wide range of organs, excluding the brain, spinal cord, bone, and bone marrow.
- Amyloidosis in CD-1 mice is more prevalent than in several other common laboratory strains and is a key factor in their mortality.