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Comparison of different methods and cell lines for isolating measles virus
D N Forthal1, J Blanding, S Aarnaes
1Department of Medicine, University of California, Irvine College of Medicine, Orange 92668.
Journal of Clinical Microbiology
|March 1, 1993
Summary
For measles virus isolation, B95-8 cells in a shell vial assay yield the highest infectivity titers. This method is recommended for efficient measles virus detection.
Area of Science:
- Virology
- Cell Biology
Background:
- Measles virus is a significant human pathogen.
- Accurate infectivity titration is crucial for viral research and diagnostics.
Purpose of the Study:
- To evaluate various methods and cell lines for determining measles virus infectivity titers.
- To identify the optimal cell line and assay for measles virus isolation.
Main Methods:
- Seven strains of measles virus were tested.
- Infectivity titers were determined using multiple assay methods.
- Various cell lines were employed for viral propagation and detection.
Main Results:
- The shell vial assay using B95-8 cells demonstrated the highest infectivity titers compared to other methods and cell lines.
- Specific infectivity titers varied across different measles virus strains and assay conditions.
Conclusions:
- B95-8 cells are highly effective for measles virus isolation.
- The shell vial assay in conjunction with B95-8 cells is the preferred method for maximizing measles virus infectivity titers.