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Expression of the La Crosse M segment proteins in a recombinant vaccinia expression system mediates pH-dependent
D R Jacoby1, C Cooke, I Prabakaran
1Department of Neurology, University of Pennsylvania Medical Center, Philadelphia 19104-6146.
Virology
|April 1, 1993
Abstract:
To study the expression of La Crosse virus (LAC) glycoproteins, G1 and G2, we constructed a cDNA copy of the open reading frame (ORF) of the middle RNA segment and expressed it in a recombinant vaccinia virus (VV.ORF). Cells infected with VV.ORF expressed G1 and G2 at the cell surface and formed syncytia with a pH profile similar to that of LAC. These experiments provide a system of studying the biological functions of the LAC glycoproteins, including processing, targeting, fusion, receptor binding, and antigenicity.