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[Herpes simplex encephalitis]

T Morishima1

  • 1Department of Pediatrics, Nagoya University School of Medicine.

No to Hattatsu = Brain and Development
|March 1, 1993
PubMed
Summary
This summary is machine-generated.

Early diagnosis of Herpes simplex encephalitis (HSE) is crucial for children. Polymerase chain reaction (PCR) assay of cerebrospinal fluid (CSF) offers a non-invasive method for detecting herpes simplex virus DNA, improving patient outcomes.

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Area of Science:

  • Neurology
  • Virology
  • Infectious Diseases

Context:

  • Herpes simplex encephalitis (HSE) presents significant mortality and morbidity, particularly in pediatric populations.
  • Effective antiviral treatments necessitate early and accurate diagnosis for improved patient outcomes.
  • Traditional diagnostic methods like brain biopsy are invasive.

Purpose:

  • To evaluate the utility of the polymerase chain reaction (PCR) assay for the early, non-invasive diagnosis of Herpes simplex encephalitis (HSE) in children.
  • To assess the detectability and serial quantification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) during the acute phase of HSE.

Summary:

  • Herpes simplex virus (HSV) DNA was detected in the cerebrospinal fluid (CSF) of all pediatric HSE patients during the acute phase using PCR.

Related Experiment Videos

  • Serial quantification of HSV DNA via PCR demonstrated a gradual decrease corresponding with antiviral therapy.
  • Viral DNA became undetectable in CSF within 3 to 18 days (mean 10.1 days) post-neurological symptom onset.

    • Impact:

    • The PCR assay serves as a valuable tool for the early and non-invasive diagnosis of HSE.
    • This diagnostic approach can lead to timely initiation of antiviral therapy, potentially reducing mortality and morbidity.
    • Facilitates improved management strategies for pediatric patients diagnosed with HSE.