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Cellular fatty acid compositions of "Achromobacter groups B and E"
B Holmes1, C W Moss, M I Daneshvar
1Identification Services Laboratory, Central Public Health Laboratory, Colindale, London, United Kingdom.
Journal of Clinical Microbiology
|April 1, 1993
Summary
Cellular fatty acid (CFA) analysis revealed that "Achromobacter groups B and E" share identical profiles. These findings support their classification as biotypes within a single new bacterial genus and species.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Chemotaxonomy
Background:
- Achromobacter groups B and E
Purpose of the Study:
- To determine the chemical relatedness of "Achromobacter groups B and E" to known bacterial taxa.
- To support previous findings on the taxonomic classification of these bacterial groups.
Main Methods:
- Cellular fatty acid (CFA) composition analysis.
- Liberation of CFAs by base hydrolysis.
- Methylation of CFAs.
- Capillary gas-liquid chromatography (GLC) for CFA profile analysis.
Main Results:
- The CFA profiles of "Achromobacter groups B and E" were identical.
- The CFA profiles of these groups were distinct from all other previously tested bacteria.
- CFA data corroborated findings from whole-cell protein analysis and DNA hybridization studies.
Conclusions:
- "Achromobacter groups B and E" represent biotypes of a single new genus and species.
- CFA composition is a reliable marker for bacterial classification and differentiation.
- This study strengthens the taxonomic placement of these bacterial groups.