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A constant flux system using the 'magnetic cell separator' (MACS) for highly efficient T-cell depletion
H D Kleine1, D Scheinichen, E Lux
1Department of Hematology and Oncology, Hannover Medical School, FRG.
Abstract:
A method for highly efficient T-cell depletion using a 'Magnetic Cell Separator' (MACS) is described. T-lymphocytes were depleted from peripheral blood samples of 37 patients with chronic lymphocytic leukemia (CLL). The flow speed in the MACS column was adjusted by a syringe-driver allowing a constant flow speed of about 100 microliters/min. The mean content of T-cells after depletion was 0.17% (S.D. +/- 0.27). The effectiveness of depletion was calculated to a mean 93.7%. There was no correlation between the proportion of CD3-positive cells before and after depletion.