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Detection of antigenemia by enzyme-linked immunosorbent assay in horses with experimental Ehrlichia risticii
R E Corstvet1, S D Gaunt, P A Karns
1Department of Veterinary Science, LSU Agricultural Center, Baton Rouge.
Abstract:
Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti-E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.