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Identification of Streptococcus mutans antigen D as the HPr component of the sugar-phosphotransferase transport
I C Sutcliffe1, S D Hogg, R R Russell
1Department of Oral Biology, Dental School, University of Newcastle upon Tyne, UK.
FEMS Microbiology Letters
|February 15, 1993
Abstract:
Extraction of Streptococcus mutans with the detergents HECAMEG and lauroyl sarcosinate preferentially extracted antigen D, previously identified as a low molecular mass wall-associated protein. Western blotting with specific antisera was used to demonstrate that this antigen is the HPr component of the sugar-phosphotransferase transport system. Non-denaturing electrophoresis indicated that HECAMEG selectively extracted only one of the two forms of HPr. It is suggested that this form of HPr may have a specific cell surface location.