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Automated technique for sampling milk from farm bulk tanks: collaborative study

V S Packard1, R E Ginn, D T Metzger

  • 1University of Minnesota, Department of Food Science and Nutrition, St. Paul 55108.

Journal of AOAC International
|March 1, 1993
PubMed
Summary

An automated milk sampling device accurately collects representative samples for composition and microbial analysis. This automated method, validated against manual sampling, is now an AOAC International first action method.

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Area of Science:

  • Agricultural Science
  • Food Science
  • Analytical Chemistry

Background:

  • Accurate milk sampling is crucial for quality assessment.
  • Traditional manual sampling can be labor-intensive and prone to variability.
  • Need for an automated, reliable method for farm bulk tank milk sampling.

Purpose of the Study:

  • To evaluate an automated, in-line mechanical sampling technique for farm bulk tank milk.
  • To compare the accuracy of automated sampling with manual methods for both milk composition and microbiological quality.
  • To assess the suitability of the automated method for adoption in routine quality control.

Main Methods:

  • A collaborative study involving an automated sampling device with a peristaltic pump.
  • Phase 1 & 2: Comparison of automated vs. manual sampling for somatic cell count, antibiotics, fat, protein, lactose, and solids-not-fat.

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  • Phase 3: Comparison of automated vs. manual sampling for total bacteria count, psychrotrophic bacteria count, and coliform count, with a sanitizing step.

    • Main Results:

    • Nested ANOVA indicated no significant difference (P = 0.05) between automated and manual sampling for composition and initial microbial analyses.
    • Automated sampling provided comparable results to manual methods for microbial analyses after implementing a sanitizing step.
    • The automated method demonstrated reliability and accuracy across various quality parameters.

    Conclusions:

    • The automated, in-line mechanical sampling technique is a valid and reliable alternative to manual sampling for farm bulk tank milk.
    • The method accurately assesses both milk composition and microbiological quality.
    • The automated sampling method has been adopted as a first action by AOAC International, indicating its acceptance for official methods.