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Related Experiment Videos

Heat-shock gene expression and cell cycle changes during mammalian embryonic development

D Walsh1, K Li, J Wass

  • 1Department of Veterinary Clinical Sciences, University of Sydney, New South Wales, Australia.

Developmental Genetics
|January 1, 1993
PubMed
Summary

Heat shock regulates cell division in early brain development. Heat shock genes (hsp) are crucial for neuroectoderm cell cycle control and recovery during embryo development.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Synchronized cell division is vital for early central nervous system (CNS) pattern formation.
  • Neural plate and neuroectoderm cells exhibit rapid division and a unique heat-shock response.
  • Heat shock can induce developmental defects and acquired thermotolerance.

Purpose of the Study:

  • To investigate the role of heat shock and heat shock genes (hsp) in neural plate development.
  • To examine the kinetics and expression of hsp genes during the cell cycle in neuroectodermal cells.
  • To understand how heat shock affects cell cycle progression and recovery.

Main Methods:

  • Whole rat embryo culture system (9.5-11.5 days).
  • Exposure to nonlethal (42°C), lethal (43°C), and thermotolerant (42°C/43°C) heat shocks.

Related Experiment Videos

  • Flow cytometry and Northern analysis to examine hsp gene expression and mRNA levels.

    • Main Results:

    • Heat shock delays cell cycle progression at G1/S and S/G2 boundaries, lengthening cell cycle time.
    • Specific hsp genes show regulated expression during different cell cycle phases (e.g., hsp 88 at G0, hsp 71 at G2+M).
    • Thermotolerant heat shock induces hsp 71 across all cell cycle phases, while lethal heat shock dramatically alters hsp expression, particularly hsp 71 in late S phase.

    Conclusions:

    • Heat shock genes (hsp) may function as critical cell cycle regulators during neuroectoderm induction and differentiation.
    • Regulated hsp expression during the cell cycle plays a significant role in the fate and recovery of neuroectoderm cells in early mammalian embryos.