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Lipid peroxidation on dialysis membranes
1Institute of Biochemistry I, Charles University, School of Medicine I, Prague, Czech Republic.
Biochemistry and Molecular Biology International
|March 1, 1993
Summary
This study found that hemodialysis (HD) increases lipid peroxidation, measured by thiobarbituric acid reacting substance (TBARS) levels. Different dialyzer membranes, like Alpha 500 and Pro500, showed varying impacts on these oxidative stress markers during HD sessions.
Area of Science:
- Nephrology
- Biochemistry
- Oxidative Stress Research
Background:
- Hemodialysis (HD) is a vital treatment for kidney failure.
- Oxidative stress, indicated by lipid peroxidation, is a concern in HD patients.
- Dialyzer membranes may influence the extent of oxidative stress during HD.
Purpose of the Study:
- To compare changes in lipid peroxidation during hemodialysis using different dialyzer membranes.
- To assess the impact of HD on thiobarbituric acid reacting substance (TBARS) levels.
- To investigate differences in oxidative stress markers between various membrane types.
Main Methods:
- Blood and plasma samples were collected from hemodialysis patients and a control group.
- Levels of total and free TBARS were measured to assess lipid peroxidation.
- TBARS concentrations were compared before, during, and after HD sessions using Alpha 500 and Pro500 membranes.
Main Results:
- HD patients exhibited significantly higher baseline TBARS levels than healthy controls.
- TBARS levels increased significantly during HD sessions on both Alpha 500 and Pro500 membranes.
- Acute increases in lipid peroxidation were observed during HD, with higher levels noted after 15 minutes on the Alpha 500 membrane compared to Pro500.
Conclusions:
- Hemodialysis significantly alters lipid peroxidation levels.
- The type of dialyzer membrane used influences the acute changes in lipid peroxidation during HD.
- Patients on regular HD treatment may experience more intensive lipid peroxidation.