Related Experiment Video
Updated: Aug 18, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
Published on: April 18, 2013
Characterization procedure for ion-selective electrode assays of magnesium activity in aqueous solutions of
R Eugster1, B Rusterholz, A Schmid
1Department of Organic Chemistry, Swiss Federal Institute of Technology (ETH), Zürich.
Abstract:
A magnesium ion-selective liquid membrane electrode based on a synthetic neutral carrier is presented. The selectivity for Mg2+ over Na+, K+, and H+ is sufficient for assays in the physiological range, but a chemometric correction of approximately 10% is still necessary for the Ca2+ interference. We optimized the membrane composition especially with respect to selectivity and accuracy by performing a new characterization procedure in which we simulated the variety of blood serum samples with 10 aqueous solutions. After a three-point calibration, these solutions were measured alternately with the middlerange calibrator for 3 min. The magnesium activity was evaluated after correcting for the calcium interference according to the Nikolsky equation. The selectivity coefficient, KpotMgCa, was itself fitted to give the minimum deviations from the assigned magnesium activities. Electrodes with an optimized membrane composition show an average deviation from theoretical activity values of about 1.6% with logKpotMgCa = -0.8.
More Related Videos
07:38Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
Published on: March 30, 2015
09:18Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
Published on: May 3, 2015
Related Concept Videos
Qualitative Analysis
For instance, group IV...
Chemical Equilibria: Systematic Approach to Equilibrium Calculations
The first step is to identify all the chemical reactions involved, The...
Potentiometry: Membrane Electrodes
Electrogravimetric Analysis: Overview
To test the completeness of the...
Electrodeposition
Electrodeposition can...
Extraction: Advanced Methods