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Related Experiment Videos

Granulocyte aggregation is edetic acid and temperature dependent

N Bizzaro1

  • 1Laboratory of Clinical Pathology, Ospedale Civile, S. Doná di Piave, Venice, Italy.

Archives of Pathology & Laboratory Medicine
|May 1, 1993
PubMed
Summary

Leukocyte agglutination, a blood cell clumping, occurred in EDTA-anticoagulated samples at room temperature. This phenomenon was linked to a plasma factor, likely IgM, in patients.

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Area of Science:

  • Hematology
  • Clinical Pathology
  • Immunology

Background:

  • Routine automated blood cell counts can reveal unexpected hematological phenomena.
  • Leukocyte agglutination is a rare event that can interfere with accurate cell counts.
  • Previous observations of leukocyte agglutination were sporadic, lacking clear causative factors.

Purpose of the Study:

  • To investigate the cause of leukocyte agglutination observed during automated blood cell counts.
  • To identify the specific conditions and factors contributing to this hematological artifact.
  • To determine the nature of the plasma factor responsible for inducing leukocyte aggregation.

Main Methods:

  • Observation of 19 cases of leukocyte agglutination over six years using a Technicon H6000 autoanalyzer.
  • Analysis of ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood samples.
  • Reproduction of agglutination by incubating patient serum/plasma with healthy donor blood; testing with anti-IgM serum.

Main Results:

  • Leukocyte agglutination exclusively occurred in EDTA-anticoagulated samples at room temperature.
  • The phenomenon was reproducible by mixing patient plasma/serum with normal blood.
  • Preincubation with anti-IgM serum prevented agglutination, implicating IgM as the causative factor.

Conclusions:

  • A plasma factor, strongly suggested to be IgM, is responsible for EDTA-dependent leukocyte agglutination.
  • The presence of this IgM factor could not be linked to specific diseases or drug usage.
  • Understanding this phenomenon is crucial for accurate interpretation of automated blood cell counts.

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