[Use of hydrazine for rapid deblocking of synthetic oligonucleotides]

N N Polushin, I N Pashkova, O G Chakhmakhcheva

|March 1, 1993

Abstract:

A rapid method for the removal of protecting groups from synthetic oligodeoxyribonucleotides, obtained by the phosphoroamidite and H-phosphonate methods, including the use of hydrazine solutions has been developed. The combination of this procedure with the application of isopropoxyacetyl N-protecting group and oxalyl ester linkage for the first nucleoside attachment to the polymer support allows to reduce to several minutes the time needed for the full oligonucleotide deprotection.

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Abstract:

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