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Semi-automated solid-phase extraction procedure for drug screening in biological fluids using the ASPEC system in
X H Chen1, J P Franke, K Ensing
1University Centre for Pharmacy, Department of Analytical Chemistry and Toxicology, Groningen, Netherlands.
Journal of Chromatography
|April 2, 1993
Abstract:
The use of a semi-automated solid-phase extraction system (ASPEC) for the screening of drugs in plasma and urine on a single mixed-mode column (Clean Screen DAU) is described. The processes of column preconditioning, sample application, column wash, pH adjustment and elution of the drugs were accomplished by the ASPEC. After off-line evaporation, the residues were injected into a wide-bore capillary gas chromatograph. The recoveries of the tested drugs were in the range of 73-96%, with relative standard deviations less than 5% at a concentration level of 2 micrograms/ml.