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Vitamin D binding protein is produced by human monocytes
A Sabbatini1, M Petrini, L Mattii
1U.O. Ematologia, Clinica Medica 1, University of Pisa, Italy.
FEBS Letters
|May 24, 1993
Summary
Vitamin D binding protein (DBP) gene expression is absent in lymphocytes but present in activated monocytes. This suggests DBP
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- The role of vitamin D binding protein (DBP) in immune cell function is not fully understood.
- Investigating DBP gene expression provides insights into its regulation and potential involvement in monocyte activation.
Purpose of the Study:
- To determine the expression pattern of the DBP gene in human monocytes and lymphocytes.
- To explore the temporal regulation of DBP gene expression during monocyte activation.
Main Methods:
- Polymerase Chain Reaction (PCR) amplification of DBP gene message.
- Southern blotting for specific DBP probe detection.
- Analysis of DBP expression in isolated monocytes, lymphocytes, and the U937 cell line.
Main Results:
- DBP gene expression was not detected in peripheral blood lymphocytes or the U937 monocyte cell line.
- DBP gene expression was identified in monocytes stimulated with lipopolysaccharide (LPS) between 6 and 10 hours post-stimulation.
Conclusions:
- DBP gene expression is specific to activated monocytes, not lymphocytes.
- The temporal expression of DBP in monocytes suggests a role in their activation by 1,25(OH)2D3.