Related Experiment Videos
Ion-pair extraction of histamine from biological fluids and tissues for its determination by high-performance liquid
D Tsikas1, R Velásquez, C Toledano
1Department of Gastroenterology and Hepatology, Hannover Medical School, Germany.
Journal of Chromatography
|April 21, 1993
Abstract:
A procedure for the selective ion-pair extraction of histamine from various biological fluids and tissues by using the ion-pairing agent bis(2-ethylhexyl)hydrogen-phosphoric acid has been developed. This procedure enabled routine determination of histamine in plasma, urine and tissue samples of humans and rats by reversed-phase high-performance liquid chromatography with fluorescence detection using o-phthaldialdehyde.