Increased type III/I collagen and alpha 1(I)/alpha 2(I) chain in a bronchopulmonary dysplastic lung

The type ratio of collagen III/I and molecule ratio of alpha 1(I)/alpha 2(I) were analysed in lungs from five infants with bronchopulmonary dysplasia (BPD) and seven control infants. All five BPD lungs showed typical characteristics, with pulmonary fibrosis and irregular cystic formation at postmortem examination. A pepsin digestion technique followed by interrupted gel electrophoresis was performed for the determination and molecular analysis of collagens III and I. The ratio of collagen types III and I were significantly increased in BPD lung (P < 0.005). The ratio of alpha 1(I) and alpha 2(I) were also significantly increased in BPD lung (P < 0.05). It is likely that collagen III is predominantly increased and that the alpha 1(I) trimer is formed in the active stage of BPD lungs.