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Cloning and functional expression of a brain G-protein-coupled ATP receptor
T E Webb1, J Simon, B J Krishek
1Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, UK.
FEBS Letters
|June 14, 1993
Abstract:
A cDNA encoding a novel member of the G-protein-coupled receptor (GCR) superfamily, an ATP receptor, has been isolated from an embryonic chick whole brain cDNA library by hybridization screening. The encoded protein has a sequence of 362 amino acids (41 kDa) and shares no more than 27% amino acid identity with any known GCR. When expressed as a complementary RNA (cRNA) in Xenopus oocytes a slowly-developing inward current was observed in response to application of ATP. The pharmacology of this expressed protein defines it as a P2Y purinoceptor.