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Related Experiment Videos

Type X collagen does not bind to matrix vesicles

S Inao1, H E Conrad

  • 1Department of Orthopaedic Surgery, Asahikawa Medical College, Japan.

[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|March 1, 1993
PubMed
Summary
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Type X collagen and matrix vesicles are released by chondrocytes. While initially appearing associated, Type X collagen

Area of Science:

  • Biochemistry
  • Cell Biology
  • Skeletal Biology

Background:

  • Hypertrophic chondrocytes play a crucial role in endochondral ossification.
  • Matrix vesicles are key initiators of mineralization in cartilage.
  • Type X collagen is a specific marker of hypertrophic chondrocytes.

Purpose of the Study:

  • To investigate the association between matrix vesicles and Type X collagen released by cultured chick embryo tibial hypertrophic chondrocytes.
  • To determine the nature of the interaction and the mechanism of retention during filtration and ultracentrifugation.

Main Methods:

  • Culturing chick embryo tibial hypertrophic chondrocytes.
  • Filtration of culture medium through various membranes (nitrocellulose, polysulfone).
  • Enzymatic treatment (pepsin, trypsin) of Type X collagen.

Related Experiment Videos

  • Ultracentrifugation of culture medium.

    • Main Results:

    • Type X collagen and matrix vesicles were co-retained on 0.1 micron nitrocellulose filters, suggesting association.
    • Varying retention ratios with different filter materials indicated selective adsorption of Type X collagen.
    • Enzymatic removal of noncollagenous extensions abolished nitrocellulose filter retention of Type X collagen.
    • Ultracentrifugation pelleted most matrix vesicles but only a small fraction of Type X collagen.

    Conclusions:

    • The quantitative retention of matrix vesicles and Type X collagen on nitrocellulose filters is due to filtration of vesicles and adsorption of collagen.
    • Type X collagen adsorption is mediated by its noncollagenous extensions.
    • Matrix vesicles and Type X collagen are not directly associated products of hypertrophic chondrocytes.