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A rapid and simple method for determining the DNA sequences of fragments inserted into vaccinia virus
N Sheng1, J Zhang, J L Whitton
1Dept. of Periodontics, University of Michigan, Ann Arbor 48109.
Biotechniques
|May 1, 1993
Abstract:
Recombinant virus vectors such as vaccinia virus, adenovirus and herpesvirus are frequently used to express a variety of foreign products. A rapid method allowing the precise identification of recombinants would be useful to confirm the nature of a newly produced recombinant and, in particular, to discriminate between recombinants bearing near-identical foreign products. Using vaccinia virus, we describe a method that in one day provides sequence analysis of the recombinant viral DNA.