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Animal models using lymphocytic choriomeningitis virus.

M von Herrath1, J L Whitton

  • 1The Scripps Research Institute, La Jolla, California, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study details methods for infecting mice with lymphocytic choriomeningitis virus (LCMV) and measuring immune responses. Protocols cover T cell and antibody detection, aiding LCMV research.

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Area of Science:

  • Virology
  • Immunology
  • Infectious Diseases

Background:

  • Lymphocytic choriomeningitis virus (LCMV) is a model pathogen for studying viral infections and immune responses.
  • Understanding host-pathogen interactions requires robust methods for infection and immune assessment.

Purpose of the Study:

  • To provide comprehensive protocols for inducing and maintaining LCMV infection in mice.
  • To describe methods for quantifying T cell and antibody responses against LCMV.
  • To offer essential support protocols for LCMV stock preparation and titer measurement.

Main Methods:

  • Induction of systemic and persistent LCMV infection in murine models.
  • Measurement of T cell responses specific to LCMV.
  • In vivo assessment of anti-LCMV immunity.
  • LCMV stock preparation and plaque assays for viral titration.
  • Enzyme-linked immunosorbent assay (ELISA) for anti-LCMV antibody detection.

Main Results:

  • Established protocols enable reliable induction of LCMV infection in mice.
  • Methods for measuring T cell and antibody responses are detailed.
  • Support protocols facilitate LCMV stock management and quantification.

Conclusions:

  • The provided protocols offer a standardized framework for LCMV research.
  • These methods are crucial for investigating viral pathogenesis and immune control of LCMV.

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