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Updated: Aug 14, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Direct sequencing of double-stranded PCR products incorporating a chemiluminescent detection procedure
A M Douglas1, A M Georgalis, B A Atchison
1Victorian Institute of Forensic Pathology, Monash University, South Melbourne, Australia.
Abstract:
A simple and reliable method is described for direct sequencing of material generated by the polymerase chain reaction (PCR). Sequencing reactions can be performed directly on PCR products without the need for purification of the template by removal of residual deoxyribonucleoside triphosphates or primers. The coupling of a chemiluminescent detection system with the use of the same primers in the initial and sequencing PCR's allows for sequencing of a number of PCR products on the one gel.
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