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Updated: Aug 14, 2026

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
Published on: December 7, 2015
[Oscillations of intracellular pH values and the character of its distribution in immunocompetent cell populations]
Abstract:
Experimental measurements of intracellular pH kinetics detect intracellular pH (pHin) rhythms with a period about an hour and an amplitude comparable with the standard deviation of pHin in cell population. It was shown that pHin oscillations were probably to affect the experimental pH-distribution pattern. The pH-distribution of the population at normal physiological state is usually close to the normal pattern. The time-course of the pHin value for the cell population was shown to have a pattern of a kind of beatings to get the normal pH-distribution. Distributions of several "beating"--functions were used to approximate an experimental pH-distribution of immunocompetent cell population.
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