Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vaccinia virus cytotoxin

J Wolstenholme, C G Woodward, R D Burgoyne

    Archives of Virology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Researchers discovered a virus-induced cytotoxin in vaccinia-infected cells. This cytotoxic factor, purified and characterized, shows specificity and toxicity to uninfected host cells, suggesting a new mechanism of viral pathogenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Revolutionizing Pediatric Neurophysiology With Magnetoencephalography.

    Psychophysiology·2026
    Same author

    Differences in neurophysiological resting-state alpha spectral events in 4-12-year-old children with prenatal exposure to alcohol relative to typically developing controls.

    Neuroscience·2025
    Same author

    First Constraints on the Epoch of Reionization Using the Non-Gaussianity of the Kinematic Sunyaev-Zel'dovich Effect from the South Pole Telescope and Herschel-SPIRE Observations.

    Physical review letters·2024
    Same author

    The XENONnT dark matter experiment.

    The European physical journal. C, Particles and fields·2024
    Same author

    Individualised Estimation of Quality-adjusted Survival Benefit and Cost-effectiveness of Proton Beam Therapy in Intermediate-stage Hodgkin Lymphoma.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2023
    Same author

    Search for New Physics in Electronic Recoil Data from XENONnT.

    Physical review letters·2022
    Same journal

    Molecular epidemiological investigation of viruses in Amur tigers in Northeast China.

    Archives of virology·2026
    Same journal

    Mining viruses in public databases unveils the diversity within the Deltaflexiviridae family.

    Archives of virology·2026
    Same journal

    Identification of novel hepaciviruses in rock pigeon (Columba livia [Gmelin, 1789]), rusty-margined flycatcher (Myiozetetes cayanensis [Linnaeus, 1766]), and Hispaniolan amazon (Amazona ventralis [Statius Muller, 1776]).

    Archives of virology·2026
    Same journal

    Structural insights into DENV-2 NS2B-NS3 protease and inhibition by glutathione-coated gold nanocluster.

    Archives of virology·2026
    Same journal

    Genomic characterization of three novel Shiga toxin-producing Escherichia coli lytic phages.

    Archives of virology·2026
    Same journal

    Molecular characterization of a novel partitivirus isolated from Fusarium solani, the causal agent of tobacco root rot.

    Archives of virology·2026
    See all related articles

    Area of Science:

    • Virology
    • Cell Biology
    • Biochemistry

    Background:

    • Vaccinia virus infection can alter host cell functions.
    • Understanding virus-host interactions is crucial for disease mechanisms.
    • Cytotoxins play a role in pathogenesis by damaging host cells.

    Purpose of the Study:

    • To identify and characterize cytotoxic factors produced during vaccinia virus infection.
    • To investigate the nature and specificity of the cytotoxic agent.
    • To explore the concept of virus-induced cytotoxins.

    Main Methods:

    • Preparation of cell extracts from vaccinia-infected and uninfected HeLa cells.
    • Centrifugation, membrane filtration, and DEAE-cellulose/Sephadex G-100 chromatography for purification.
    • Assays for cytotoxicity, molecular weight determination, enzyme inactivation (trypsin, ribonuclease), and immunosorbent chromatography for virus specificity.

    Related Experiment Videos

    Main Results:

    • A cytotoxic factor was isolated from vaccinia-infected HeLa cells, toxic to uninfected cells.
    • The factor, purified 100-fold, has a molecular weight of 30-100,000 daltons, is acidic, and inactivated by trypsin but not ribonuclease.
    • Virus specificity was confirmed, and similar factors were found in other infected cell lines (HEp-2, RK, W-K).

    Conclusions:

    • A virus-specific cytotoxic factor is produced during vaccinia virus infection.
    • This cytotoxin exhibits properties suggesting it is a protein.
    • The findings support the concept of virus-induced cytotoxins acting within host cells.