Related Experiment Video
Updated: Aug 15, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Published on: March 31, 2012
A plethora of GTPases, large and small
1Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City 66160.
Abstract:
It has become apparent recently that a variety of small GTPases (small GTP-binding proteins or smgs), related to the oncogene ras, are involved in many cellular functions, especially membrane traffic and cytoskeleton regulation. This review will summarize some of the major themes that are emerging, focusing on the presence and possible functions of these smgs in the gastrointestinal tract. In addition, new cellular locations and functions for heterotrimeric G-proteins, previously believed to be solely associated with plasma membrane receptors, are becoming evident and will be discussed here.
Related Concept Videos
GTPases and their Regulation
Large G-proteins, also known...
Coat Assembly and GTPases
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
GTPases and their Regulation
Large G-proteins, also known...
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Activation and Inactivation of G Proteins

