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Glucose transport during cell cycle in IM9 lymphocytes
G B Bushart1, U Vetter, W Hartmann
1Abteilungen für Paediatrie I und II, Universität Ulm, Germany.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|April 1, 1993
Summary
Hexose uptake in IM9 lymphocytes shows specific kinetics and is not affected by insulin or IGF-I. Glucose transport significantly increases during the S-phase of the cell cycle.
Area of Science:
- Cell Biology
- Biochemistry
- Endocrinology
Background:
- The IM9 cell line is a human B-lymphoblastoid cell line.
- Understanding glucose transporter kinetics is crucial for cellular metabolism research.
- Insulin and Insulin-like Growth Factor I (IGF-I) are key metabolic regulators.
Purpose of the Study:
- To analyze hexose uptake kinetics in the IM9 lymphocyte cell line.
- To investigate the responsiveness of IM9 cells to insulin and IGF-I.
- To determine the cell cycle dependency of hexose transport in IM9 lymphocytes.
Main Methods:
- Kinetics analysis of 2-deoxy-D-[3H]glucose uptake.
- Treatment with physiological concentrations of insulin and IGF-I.
- Cell cycle phase enrichment using counterflow elutriation for 2-deoxy-D-[3H]glucose transport investigation.
Main Results:
- A Michaelis constant (Km) of 0.76 mM and a maximum velocity (Vmax) of 14.9 nMol/min/mg protein were determined for 2-deoxy-D-[3H]glucose uptake.
- Hexose uptake in IM9 lymphocytes was not significantly altered by insulin or IGF-I.
- Hexose uptake per cell more than doubled during the S-phase, returning to G1-phase levels in G2-phase cells.
Conclusions:
- IM9 lymphocytes possess glucose transporter kinetics similar to erythrocyte and brain types.
- Insulin and IGF-I do not modulate hexose uptake in this lymphocyte cell line.
- Hexose transport is cell cycle-dependent, with a marked increase during the S-phase.