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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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Antibody-mediated suppression of tumor growth. I. Suppression by murine IgG1 isolated from alloantiserum

R J Johnson, G R Pasternack, H S Shin

    Journal of Immunology (Baltimore, Md. : 1950)
    |February 1, 1977
    PubMed

    The tumor suppressive activity of murine IgG1 antibody was studied in vivo. IgG1 was isolated from hyperimmune alloantisera against a murine lymphoma, 6C3HED, by absorption with heat-killed, formalinized Staphylococcus aureus. Cowan strain I, followed by DEAE ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The isolated IgG1, which had no detectable IgM, IgA, IgG2a, IgG2b or IgG3, could suppress the growth of the tumor. In addition, DEAE and Sephadex G-200 column profiles of the in vivo tumor suppressive activity showed good correlation with the profiles of total IgG1 immunoglobulin and anti-tumor antibody assayed for IgG1 heavy chains and kappa light chains. The IgG1 tumor suppressive activity was not diminished after heating at 56 degrees C for 30 min.

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