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Crystallization and preliminary X-ray diffraction studies of Theiler's virus (GDVII strain)
K S Toth1, C X Zhang, H L Lipton
1Department of Microbiology, University of Alabama, Birmingham 35294.
Journal of Molecular Biology
|June 20, 1993
Summary
Theiler's murine encephalomyelitis virus (TMEV) GDVII strain was crystallized. These crystals diffracted X-rays, enabling high-resolution structural analysis for neurological disease research.
Area of Science:
- Virology
- Structural Biology
- Neuroscience
Background:
- Theiler's murine encephalomyelitis virus (TMEV) is a picornavirus.
- TMEV-infected mice are models for human neurological diseases like multiple sclerosis.
Purpose of the Study:
- To crystallize the GDVII strain of TMEV.
- To assess the suitability of GDVII crystals for high-resolution structure determination.
Main Methods:
- Hanging drop technique for TMEV crystallization.
- Synchrotron X-ray diffraction analysis of crystals.
Main Results:
- Monoclinic crystals of GDVII strain TMEV were successfully prepared.
- Crystals diffracted X-rays to at least 3.0 A resolution.
- Unit cell dimensions and molecular mass suggest one virus particle per asymmetric unit with 60-fold non-crystallographic redundancy.
Conclusions:
- The GDVII crystal form is suitable for high-resolution structure determination.
- This facilitates understanding TMEV structure and its role in neurological diseases.