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Adenovirus isolations from patients with conjunctivitis in Thuringia
T Adrian1, H Brunnemann, R Wigand
1Institut für Virologie und Seuchenhygiene, Medizinische Hochschule, Hannover, Germany.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|February 1, 1993
Summary
This study identified 57 adenoviruses (AVs) from patients with conjunctivitis, with AV8 being the most common. DNA analysis revealed distinct genetic profiles for AV9 and AV17/H29 strains, aiding in their classification.
Area of Science:
- Ophthalmology
- Virology
- Molecular Biology
Background:
- Adenoviruses (AVs) are a common cause of infectious conjunctivitis.
- Understanding the genetic diversity of circulating AV strains is crucial for diagnosis and management.
Purpose of the Study:
- To identify and genetically characterize adenoviruses isolated from patients with (kerato) conjunctivitis.
- To analyze the genetic relatedness of different AV strains using DNA restriction analysis.
Main Methods:
- Isolation and identification of 57 adenoviruses from patient samples collected between 1979 and 1986.
- DNA restriction endonuclease analysis using five enzymes.
- Physical mapping of restriction site alterations for specific enzymes and AV variants.
Main Results:
- Adenoviruses 8, 7, 3, 17/H29, 9, 2, and 10 were identified, with AV8 being the most prevalent.
- Three intermediate AV17/H29 strains were found to be genetically identical.
- Two AV9 strains showed variations from the prototype strain in two enzymes, with alterations mapped.
- An AV8 variant with a HindIII alteration was also identified and mapped.
Conclusions:
- The study provides a detailed genetic characterization of adenoviruses causing conjunctivitis during the specified period.
- DNA restriction analysis is effective in differentiating and classifying AV strains.
- Identified genetic variations in AV9 and AV8 strains highlight the importance of ongoing molecular surveillance.