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Purification and characterization of (S)-1,3-butanediol dehydrogenase from Candida parapsilosis
H Yamamoto1, A Matsuyama, Y Kobayashi
1Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan.
Bioscience, Biotechnology, and Biochemistry
|September 1, 1995
Abstract:
(S)-1,3-Butanediol (BDO) oxidizing enzyme was purified from Candida parapsilosis IFO 1396, which could produce (R)-1,3-BDO from the racemate. The purified enzyme was an NAD(+)-dependent secondary alcohol dehydrogenase that oxidized (S)-1,3-BDO to 4-hydroxy-2-butanone stereo-specifically.