Related Experiment Video
Updated: Aug 10, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
[Proteinases activated by gamma irradiation in the nuclei of rat peripheral blood leukocytes]
Abstract:
The nuclei of the peripheral blood leucocytes of rats displayed proteolytic activity (Ca-dependent with pH optimum 8.5 and active at alkaline pH), whose level increases under the influence of gamma-irradiation. These proteases were partially purified and identified. It was shown using gel chromatography, affinity chromatography with casein-sepharose and enzyme-electrophoresis that these proteolytic activities are related to one protein with a molecular mass of 24 kDa or a complex of proteins that do not cleave under the given experimental conditions. The both protease activities are predominantly blocked by inhibitors of cysteine proteases.
More Related Videos
08:18Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
07:40Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021