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Methods for urinary testosterone analysis

E Venturelli1, A Cavalleri, G Secreto

  • 1National Cancer Institute, Endocrine Unit, Milano, Italy.

Journal of Chromatography. B, Biomedical Applications
|September 15, 1995
PubMed
Summary
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Accurate urinary testosterone measurement involves hydrolysis, extraction, and purification. A recommended method uses enzymatic hydrolysis, C18/NH2 cartridge extraction, HPLC cleanup, and GC or RIA quantification for reliable results.

Area of Science:

  • Endocrinology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Urinary testosterone measurement is crucial for diagnosing hormonal imbalances and monitoring endocrine function.
  • Accurate quantification requires overcoming challenges in sensitivity and specificity due to complex urine matrices.
  • Existing methods often involve multiple, time-consuming steps with varying degrees of success.

Purpose of the Study:

  • To provide a comprehensive overview of techniques for urinary testosterone analysis.
  • To outline a reliable, multistep procedure for sensitive and specific testosterone dosage.
  • To compare advantages and disadvantages of different analytical approaches.

Main Methods:

  • Enzymatic hydrolysis using Helix pomatia juice.

Related Experiment Videos

  • Solid-phase extraction with C18 and NH2 cartridges.
  • High-performance liquid chromatography (HPLC) for cleanup.
  • Quantification via gas chromatography (GC) or radioimmunoassay (RIA).

    • Main Results:

    • The proposed method integrates enzymatic hydrolysis, dual-cartridge solid-phase extraction, and HPLC cleanup.
    • This procedure enhances both sensitivity and specificity in urinary testosterone determination.
    • Both GC and RIA are presented as viable quantification techniques post-cleanup.

    Conclusions:

    • A standardized, multistep protocol is essential for reproducible urinary testosterone analysis.
    • The suggested method offers a robust approach for accurate hormone level determination.
    • This analytical strategy supports clinical and research applications requiring precise testosterone measurement.