Precise large deletions by the PCR-based overlap extension method
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Updated: Nov 6, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Published on: January 3, 2015
1Department of Microbiology, University of Tennessee, Knoxville 37996-0845, USA.
Abstract:
The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
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