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An ultrasensitive immunoassay for prostate-specific antigen based on conventional colorimetric detection
M J Khosravi1, A Papanastasiou-Diamandi, J Mistry
1Diagnostic Systems Laboratories (Canada) Inc., Toronto, Ontario.
Clinical Biochemistry
|August 1, 1995
Summary
An ultrasensitive immunoassay for prostate-specific antigen (PSA) was developed. This assay is highly effective for monitoring PSA levels in prostate cancer patients after surgery.
Area of Science:
- Biochemistry
- Immunology
- Oncology
Background:
- Prostate-specific antigen (PSA) is a key biomarker for prostate cancer.
- Accurate and sensitive detection of PSA is crucial for patient monitoring, especially post-treatment.
Purpose of the Study:
- To develop and validate an ultrasensitive immunoassay for serum PSA.
- To assess the utility of this assay for post-surgical monitoring in prostate cancer patients.
Main Methods:
- Development of a one-step immunoassay utilizing a polyclonal antibody and a horseradish peroxidase-labeled monoclonal antibody.
- Enzymatic activity monitoring via hydrogen peroxide/tetramethylbenzidine substrate and spectrophotometric quantification.
Main Results:
- Achieved a low detection limit of 0.003 µg/L and a biological detection limit of 0.009 µg/L.
- Demonstrated good assay precision (CVs 8.2-10.5%), recovery (104%), and dilution linearity.
- Serum PSA levels were below 0.012 µg/L in most post-prostatectomy patients (53.8%).
Conclusions:
- The developed immunoassay is highly sensitive and suitable for monitoring PSA levels.
- This assay is well-suited for the post-surgical surveillance of prostate cancer patients.