Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

micF RNA is a substrate for RNase E

M Schmidt1, N Delihas

  • 1Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook 11794-5222, USA.

FEMS Microbiology Letters
|November 15, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and histological features retain their prognostic impact under interferon therapy of CML: a pilot study.

American journal of hematology·1995
Same author

New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.

Gene·1995
Same author

Rapid and persistent desensitization of m3 muscarinic acetylcholine receptor-stimulated phospholipase D. Concomitant sensitization of phospholipase C.

The Journal of biological chemistry·1995
Same author

cDNA analysis of the mite allergen Lep d 1 identifies two different isoallergens and variants.

FEBS letters·1995
Same author

Functional characterization of the higher plant chloroplast chaperonins.

The Journal of biological chemistry·1995
Same author

The nuclear membrane-associated honeycomb structure of the unicellular organism Amoeba proteus: on the search for homologies with the nuclear lamina of metazoa.

European journal of cell biology·1995
Same journal

Genetic basis of R-phase H:z27 antigen expression and phylogenetic relationships of Salmonella enterica serovar Senftenberg.

FEMS microbiology letters·2026
Same journal

Chitin metabolism enhancement by β-N-acetyl-D-glucosaminyl-(1,4)-D-glucosamine in Vibrio is associated with chitin disaccharide transporter.

FEMS microbiology letters·2026
Same journal

The Type III Secretion System plasmid pPHDPT3 of Photobacterium damselae subsp. piscicida is stable in Australian isolates due to conserved non-repetitive genomic architecture.

FEMS microbiology letters·2026
Same journal

Multi-copy aiiA genes encoding quorum-quenching enzymes in Bacillus thuringiensis: identification and functional characterization of the novel AHL-lactonase, AiiA2.

FEMS microbiology letters·2026
Same journal

Recombination mapping identifies a commensal Neisseria subflava origin of mosaic penA 60.001 allele in Neisseria gonorrhoeae.

FEMS microbiology letters·2026
Same journal

Long vs. short read sequencing for microbial ecology of sedimentary environments: a case study from Lake Arnon, Switzerland.

FEMS microbiology letters·2026
See all related articles

Ribonuclease E (RNase E) cleaves micF RNA, an antisense RNA in Escherichia coli. This study identifies specific cleavage sites and sequence patterns, aiding in understanding RNase E function in RNA processing.

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Ribonuclease E (RNase E) is a key enzyme in Escherichia coli, essential for messenger RNA (mRNA) decay and RNA processing.
  • While RNase E's role is established, its precise substrate recognition and cleavage site specificities remain incompletely understood.

Purpose of the Study:

  • To investigate micF RNA as a substrate for RNase E.
  • To map the specific cleavage sites of RNase E on micF RNA.
  • To define a generalized recognition and cleavage pattern for RNase E.

Main Methods:

  • In vitro enzymatic assays using purified RNase E and micF RNA.
  • High-resolution mapping of RNA cleavage sites.

Main Results:

Related Experiment Videos

  • MicF RNA was confirmed as a substrate for RNase E in vitro.
  • Two primary cleavage sites on micF RNA were identified and mapped.
  • Cleavage sites were consistently found within a UA/UUU sequence context.
  • These sites are located upstream of stem-loop structures in micF RNA.

Conclusions:

  • The findings provide specific insights into RNase E's substrate recognition mechanisms.
  • A generalized pattern for RNase E cleavage, involving specific sequence contexts and proximity to stem-loops, is proposed.
  • This work contributes to a deeper understanding of RNA processing and decay pathways in E. coli.