Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Homologous RNA recombination in brome mosaic virus: AU-rich sequences decrease the accuracy of crossovers

P D Nagy1, J J Bujarski

  • 1Plant Molecular Biology Center, Northern Illinois University, De Kalb 60115, USA.

Journal of Virology
|January 1, 1996
PubMed
Summary

AU sequences in brome mosaic virus RNA facilitate imprecise homologous recombination. Extending these AU sequences significantly increases imprecise crossovers, suggesting a role in generating genetic diversity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Next generation sequencing reveals packaging of host RNAs by brome mosaic virus.

Virus research·2018
Same author

Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA synthesis.

Plant molecular biology·2013
Same author

Emerging picture of host chaperone and cyclophilin roles in RNA virus replication.

Virology·2011
Same author

Enhancement of RNA synthesis by promoter duplication in tombusviruses.

Virology·2003
Same author

Mechanism of DI RNA formation in tombusviruses: dissecting the requirement for primer extension by the tombusvirus RNA dependent RNA polymerase in vitro.

Virology·2002
Same author

The RNA replication enhancer element of tombusviruses contains two interchangeable hairpins that are functional during plus-strand synthesis.

Journal of virology·2002

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Brome mosaic virus (BMV) is a tripartite positive-stranded RNA virus.
  • Understanding homologous recombination is crucial for viral evolution and genetic engineering.

Purpose of the Study:

  • To determine sequence requirements for imprecise (aberrant) homologous recombination in BMV.
  • To investigate the role of specific AU-rich sequences in recombination fidelity.

Main Methods:

  • Utilized BMV RNA2 and RNA3 mutants with varying lengths of a specific AU-rich sequence.
  • Analyzed precise and imprecise homologous recombination products.
  • Quantified recombination frequencies based on sequence alterations.

Main Results:

Related Experiment Videos

  • A 6-nt UUAAAA (AU) sequence supported both precise and imprecise recombination.
  • Extending the AU sequence length increased imprecise crossover frequency significantly.
  • Imprecise recombinants showed nucleotide substitutions, insertions, deletions, or duplications near the AU sequence.
  • Deletion of the AU sequence resulted exclusively in precise recombinants.

Conclusions:

  • AU sequences act as facilitators for imprecise homologous recombination in BMV.
  • Misannealing within AU sequences likely explains insertions/deletions.
  • Replicase errors may account for nucleotide substitutions and non-templated nucleotides.