RAD58 (XRS4)--a new gene in the RAD52 epistasis group
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O V Chepurnaya1, S A Kozhin, V T Peshekhonov
1Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Academy of Sciences of Russia, Gatchina, Russia.
Abstract:
The RAD58 (XRS4) gene of Saccharomyces cerevisiae has been previously identified as a DNA repair gene. In this communication, we show that RAD58 also encodes an essential meiotic function. The spore inviability of rad58 strains is not rescued by a spo13 mutation. The rad50 mutation suppresses spore inviability of a spo13 rad58 strain suggesting that RAD58 acts after RAD50 in meiotic recombination. The rad58-4 mutation does not prevent mitotic recombination events. Haploid rad58 cells fail to carry out G2-repair of gamma-induced lesions, whereas rad58/rad58 diploids are able to perform some diploid-specific repair of these lesions.