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Peritoneal exudate cells. IV. Characterization of colony forming cells
Abstract:
The adherence, phagocytic activity and buoyant density of mouse peritoneal exudate colony forming units (CFU-PE) were investigated. There was a significant enrichment in the proportion of CFU-PE in the adherent cells population, defined as cells adhering to a plastic surface within 30 minutes of incubation. The phagocytic activity of CFU-PE was studied by incubating exudate cells with iron particles for 45 minutes. The cells were then separated into phagocytic and non-phagocytic cell fractions by passing the incubation mixture through a magnetic field. A significant enrichment of CFU-PE was seen in the phagocytic cell fraction. When exudate cells were fractionated in a Ficoll discontinuous density gradient, more than 88% of CFU-PE were recovered at the 16/18% and 18/20% interfaces. It is concluded that CFU-PE are adherent cells, have strong phagocytic activity and have a buouant density between 1.0562 and 1.0703. When bone marrow cells were studied by these techniques, the committed stem cells for both granulocytes and marcophages (CFU-C) were enriched in both non-adherent cell and non-phagocytic cells populations. In the Ficoll density gradient, CFU-C banded at a heavier density region than CFU-PE.
Insights
Mouse peritoneal exudate colony forming units (CFU-PE) are adherent, phagocytic cells with a buoyant density between 1.0562 and 1.0703, distinct from bone marrow stem cells.
Area of Science:
- Hematology
- Cell Biology
- Immunology
Background:
- Colony forming units (CFU) are crucial for hematopoiesis.
- Characterizing CFU-PE is essential for understanding myeloid cell development.
- Previous studies have not fully elucidated CFU-PE properties.
Purpose of the Study:
- To investigate the adherence, phagocytic activity, and buoyant density of mouse peritoneal exudate colony forming units (CFU-PE).
- To compare the characteristics of CFU-PE with committed stem cells for granulocytes and macrophages (CFU-C).
Main Methods:
- Assessing cell adherence to plastic surfaces within 30 minutes.
- Quantifying phagocytic activity using iron particles and magnetic separation.
- Fractionating cells using Ficoll discontinuous density gradients.
Main Results:
- CFU-PE were significantly enriched in adherent cell populations.
- CFU-PE showed significant enrichment in the phagocytic cell fraction.
- Over 88% of CFU-PE were recovered at specific density interfaces (16/18% and 18/20%) in Ficoll gradients.
- Committed stem cells (CFU-C) were enriched in non-adherent, non-phagocytic populations and banded at a heavier density than CFU-PE.
Conclusions:
- CFU-PE are characterized as adherent cells with strong phagocytic activity.
- The buoyant density of CFU-PE was determined to be between 1.0562 and 1.0703.
- CFU-PE exhibit distinct cellular properties compared to CFU-C, indicating different developmental pathways.