Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for detection of IgG rheumatoid factor

C M Riera, A Risemberg, A Strusberg

    Journal of Immunological Methods
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Autoimmune prostatitis: state of the art.

    Scandinavian journal of immunology·2007
    Same author

    [Induction of primary immune response by rat dendritic cells].

    Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina)·2003
    Same author

    Identification of a CD8alpha(+) dendritic cell subpopulation in rat spleen and evaluation of its OX-62 expression.

    Clinical immunology (Orlando, Fla.)·2001
    Same author

    Modulation of arachidonic acid turnover in macrophages by cadmium.

    Toxicology letters·2001
    Same author

    Identification of rat prostatic steroid binding protein (PSBP) as an immunosuppressive factor.

    Journal of reproductive immunology·2001
    Same author

    Molecular mechanisms implicated in galectin-1-induced apoptosis: activation of the AP-1 transcription factor and downregulation of Bcl-2.

    Cell death and differentiation·2000

    A new, rapid method accurately detects IgG rheumatoid factor (RF) in patient samples. This simple latex agglutination test correlates with disease severity in rheumatoid arthritis patients.

    Area of Science:

    • Immunology
    • Rheumatology
    • Clinical Chemistry

    Background:

    • Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation.
    • Rheumatoid factor (RF), particularly immunoglobulin G (IgG) RF, is a key biomarker in RA diagnosis and prognosis.
    • Accurate and rapid detection of IgG RF is crucial for patient management.

    Purpose of the Study:

    • To describe a simplified and rapid method for detecting IgG rheumatoid factor (RF).
    • To evaluate the efficacy of the new method by comparing it with a conventional technique.
    • To investigate the clinical significance of IgG RF in synovial fluid for rheumatoid arthritis patients.

    Main Methods:

    • A novel technique involving direct incubation with 0.1 M 2-mercaptoethanol for 2 hours.

    Related Experiment Videos

  • Detection of human anti-IgG antibodies using a latex agglutination test.
  • Comparison with a conventional method using sequential treatment with 2-mercaptoethanol and iodoacetamide over 24 hours.

    • Main Results:

    • The new method demonstrated comparable results to the conventional 24-hour treatment protocol.
    • Similar detection rates for IgG RF were observed between the rapid and conventional methods.
    • A significant correlation was found between IgG RF presence in synovial fluid and severe, invalidating forms of rheumatoid arthritis.

    Conclusions:

    • The described method offers a simple, rapid, and effective approach for IgG RF detection in sera and joint fluid.
    • This technique provides reliable results comparable to established methods.
    • Elevated IgG RF in synovial fluid may indicate a more severe clinical prognosis in rheumatoid arthritis patients, aiding in clinical decision-making.