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Positive and negative roles for cdc10 in cell cycle gene expression
C J McInerny1, P J Kersey, J Creanor
1Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Nucleic Acids Research
|December 11, 1995
Summary
The cdc10-C4 mutant in fission yeast causes overexpression of cell cycle genes. This suggests the DSC1Sp/MBF complex represses and activates gene expression during the cell cycle.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- The cdc10+ gene is crucial for the DSC1Sp/MBF transcription complex.
- This complex regulates cell-cycle gene expression via MCB elements.
- Unregulated gene expression is linked to cell cycle progression.
Purpose of the Study:
- To investigate the properties of the cdc10-C4 mutant in Schizosaccharomyces pombe.
- To understand the role of the DSC1Sp/MBF complex in gene regulation.
- To elucidate the function of the cdc10 protein in cell cycle control.
Main Methods:
- Characterization of the cdc10-C4 mutant phenotype.
- Analysis of MCB-regulated gene expression levels.
- Overexpression of a specific cdc10 protein region.
Main Results:
- The cdc10-C4 mutant exhibits cold-sensitive overexpression of MCB-regulated genes.
- Loss of function, not alteration, of cdc10-C4 underlies unregulated gene expression.
- Overexpression of a cdc10 region induces G1 delay and reduces MCB gene overexpression.
Conclusions:
- The DSC1Sp/MBF complex plays a dual role in repressing and activating MCB gene expression.
- The cdc10 protein is essential for proper cell cycle-regulated gene expression.
- The cdc10-C4 mutation leads to a loss of regulatory function, impacting the cell cycle.