Large scale collection of viable infective larvae of Loa loa

A simple method to obtain large numbers of viable infective larvae (L3) of the human filarial parasite Loa loa in an endemic country is described: wild vector flies (Chrysops silacea and C. dimidiata) were caught in large numbers, sealed into pockets of mosquito netting, gently crushed on a glass plate and incubated in a funnel with medium ("Baermann technique"). 91-94% of the L3 actively left the crushed flies and accumulated in the funnel tip after 15 (80%) to 45 minutes (95%). During 111 catching days over a 12 month period, a single fly collector provided approximately 13,000 flies and 23,500 L3. The method is also considered useful for assessing loiasis-transmission, for example before and after mass treatment campaigns.