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Enhanced expression of TGF-beta 1 by peritoneal macrophages in CAPD patients
1National Institute of Kidney, Sun Yat-sen University of Medical Sciences, Guangzhou, People's Republic of China.
Abstract:
Peritoneal macrophages are the predominant cells in peritoneal dialysate. To clarify the role of the macrophage in fibrosis of the peritoneum in long-term continuous ambulatory peritoneal dialysis (CAPD) patients, we studied the transforming growth factor beta (TGF-beta 1) mRNA expression by peritoneal macrophages from peritoneal dialysis patients. Macrophages were obtained when the catheter was inserted and one month later. TGF-beta 1 mRNA was measured by slot-blot hybridization using a cDNA probe. The result was that TGF-beta 1 expression was significantly higher in macrophages from peritoneal dialysate than that of macrophages obtained during catheter insertion. This suggests that peritoneal macrophages play a role in the pathogenesis of fibrosis of peritoneum in peritoneal dialysis patients. It also suggests that peritoneal macrophages in dialysate have been somewhat activated.
Insights
Peritoneal macrophages in dialysis fluid show increased transforming growth factor beta 1 (TGF-β1) mRNA. This indicates activated macrophages contribute to peritoneal fibrosis in continuous ambulatory peritoneal dialysis (CAPD) patients.
Area of Science:
- Nephrology
- Cell Biology
- Immunology
Background:
- Peritoneal macrophages are key cells in peritoneal dialysate.
- Peritoneal fibrosis is a complication in long-term continuous ambulatory peritoneal dialysis (CAPD).
Purpose of the Study:
- To investigate the role of peritoneal macrophages in peritoneal fibrosis.
- To measure transforming growth factor beta 1 (TGF-β1) mRNA expression in peritoneal macrophages from CAPD patients.
Main Methods:
- Macrophages were isolated from peritoneal dialysate and during catheter insertion.
- Transforming growth factor beta 1 (TGF-β1) mRNA levels were quantified using slot-blot hybridization and a cDNA probe.
Main Results:
- TGF-β1 mRNA expression was significantly elevated in macrophages from peritoneal dialysate compared to those from catheter insertion.
- This suggests peritoneal macrophages in dialysate are activated.
Conclusions:
- Peritoneal macrophages play a role in the pathogenesis of peritoneal fibrosis in CAPD patients.
- Activated peritoneal macrophages in dialysate may contribute to fibrosis development.